Cmb Plasmids

Lecture 2 Bacterial Plasmids What be plasmid DNAs, and why are they so useful? [pic] What is a plasmid? |  |A plasmid is an extra-chromosomal element, a great deal a circular DNA. The plasmids we exit use in this break| | |typically bind 3 important elements: | | |A re-create site (a place to enclose foreign DNAs) | | |An origin of replica | | |A selectable marker constituent (e.g. resistance to ampicillin) | |Cloning sites |A cloning site is not required at all, exactly it sure is nice to have! What I mean by cloning site is a | | |place where the DNA rat be digested by circumstantial restriction enzymes - a demonstrate of entry or abbreviation for | | |genetic engineering work. This is a matter we will be discussing in great expatiate at a by and by point.

For | | |now, think of the following sheath: Suppose you are very thirsty and you buy a grass of beer. Does it | | |occur to you that cardinal end of the can (the round top) is designed so that you can circularize it easily? If y! ou | | |bought a can of beer with 2 bottom ends and no top, you would have a hard prison term confuseing it! Its the | | |same way with plasmids. You can have a plasmid with lots of terrific features, merely you might miss an | | |easy way of stoolting it open with restriction enzymes. All this blither is making me thirsty, and now I | | |believe Ill have that drink! | |Origins of replication:...If you want to get a full essay, place it on our website: OrderEssay.net

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